Differential Expression Analysis for RNA-Seq Data
نویسندگان
چکیده
منابع مشابه
Differential Expression Analysis for RNA-Seq Data
RNA-Seq is increasingly being used for gene expression profiling. In this approach, next-generation sequencing (NGS) platforms are used for sequencing. Due to highly parallel nature, millions of reads are generated in a short time and at low cost. Therefore analysis of the data is a major challenge and development of statistical and computational methods is essential for drawing meaningful conc...
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3 RNA–Seq data preprocessing 2 3.1 Creation of a sample metadata table . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 3.2 Quality control commands . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 3.3 Alignment of reads . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 3.4 Sorting and in...
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Background Differential expression analysis of RNA sequencing (RNA-seq) data typically relies on reconstructing transcripts or counting reads that overlap known gene structures. Previously we introduced an intermediate approach called differentially expressed region (DER) finder that seeks to identify contiguous regions of the genome showing differential expression signal at single base resolut...
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MOTIVATION RNA-Seq is a novel technology that provides read counts of RNA fragments in each gene, including the mapped positions of each read within each gene. Besides many other applications it can be used to detect differentially expressed genes. Most published methods collapse the position-level read data into a single gene-specific expression measurement. Statistical inference proceeds by m...
متن کاملBootstrap-based differential gene expression analysis for RNA-Seq data without replicates
Correspondence: [email protected] Computer Science & Engineering Department, University of Connecticut, 06269 Storrs, CT, USA Full list of author information is available at the end of the article †Equal contributor Abstract A major application of RNA-Seq is to perform differential gene expression analysis. Many tools exist to analyze differentially expressed genes in the presence of biologi...
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ژورنال
عنوان ژورنال: ISRN Bioinformatics
سال: 2012
ISSN: 2090-7346
DOI: 10.5402/2012/817508